Stem Cell Research (Apr 2022)

Establishment of an induced pluripotent stem cell line (JTUi003-A) from a patient with otosclerosis

  • Yuanping Xiong,
  • Ying Wang,
  • Maoxiang Xu,
  • Yaoqian Liu,
  • Hongqun Jiang,
  • Zhuang Jiang,
  • Jiping Wang

Journal volume & issue
Vol. 60
p. 102666

Abstract

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Otosclerosis is characterized by abnormal bone remodeling in the osseous labyrinth and progressive hearing loss. Although the etiology of otosclerosis is not fully understood, both environmental and genetic factors play important roles in its pathogenesis. Here, we generated an induced pluripotent stem cell line using episomal plasmid vectors from the peripheral blood mononuclear cells of a 48-year-old male with otosclerosis. The morphology and karyotype of the cells were normal. The expression of pluripotency markers was verified by mRNA and protein levels; the pluripotency state of the cell line was verified by successful differentiation into all three germ layers.