Uji degradasi selulosa dari jamur tanah hutan bekas terbakar Wanariset-Semboja, Kalimantan Timur

Abstract

In order to know the effect of isolation method on the occurrence and capability of soil fungi to degrade cellulose, a study wasconducted in an over-burned forest in Wanariset-Semboja, East Kalimantan. Soil fungi were isolated using three isolation methods:incubation at 45 ° C, treatment with 50% ethanol for 15 minutes, and heat treatment at 70 ° C for 15 minutes. Plates for heat incubationand for other methods were incubated at 45 ° C and 27 ° C for three days, respectively. Cellulose degradation test of isolated fungi wasexamined using Carboxy Methyl Cellulose (CMC) media. Results showed that isolation method affected diversity and population of soilfungi. Heat treatment at 70 ° C for 15 minutes appeared to have highest diversity and population of soil fungi. Eupenicillium javanicumvar javanicum (van Veyma) Stolk & Scott, Talaromyces byssoclamydoides Stolk & Samson, T. flavus (Klocker) Stolk & Samson,T. stipitatus C.R. Benjamin, and Penicillium argillaceum Stolk et al. were dominant in an over-burned forest in Wanariset-Semboja,East Kalimantan. Twenty-one isolated fungi degraded cellulose.

Keywords

• cellulose degradation
• diversity
• population
• soil fungi