Bioethanol in Biofuels Checked by an Amperometric Organic Phase Enzyme Electrode (OPEE) Working in “Substrate Antagonism” Format

Mauro Tomassetti; Gabriele Spuri Capesciotti; Riccardo Angeloni; Elisabetta Martini; Luigi Campanella

doi:10.3390/s16091355

Sensors (Aug 2016)

Bioethanol in Biofuels Checked by an Amperometric Organic Phase Enzyme Electrode (OPEE) Working in “Substrate Antagonism” Format

Mauro Tomassetti,
Gabriele Spuri Capesciotti,
Riccardo Angeloni,
Elisabetta Martini,
Luigi Campanella

Affiliations

Mauro Tomassetti: Department of Chemistry, University of Rome “Sapienza”, P.le A. Moro 5, Rome 00185, Italy
Gabriele Spuri Capesciotti: Department of Chemistry, University of Rome “Sapienza”, P.le A. Moro 5, Rome 00185, Italy
Riccardo Angeloni: Department of Chemistry, University of Rome “Sapienza”, P.le A. Moro 5, Rome 00185, Italy
Elisabetta Martini: Department of Chemistry, University of Rome “Sapienza”, P.le A. Moro 5, Rome 00185, Italy
Luigi Campanella: Department of Chemistry, University of Rome “Sapienza”, P.le A. Moro 5, Rome 00185, Italy

DOI: https://doi.org/10.3390/s16091355
Journal volume & issue: Vol. 16, no. 9
p. 1355

Abstract

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The bioethanol content of two samples of biofuels was determined directly, after simple dilution in decane, by means of an amperometric catalase enzyme biosensor working in the organic phase, based on substrate antagonisms format. The results were good from the point of view of accuracy, and satisfactory for what concerns the recovery test by the standard addition method. Limit of detection (LOD) was on the order of 2.5 × 10−5 M.

Published in Sensors

ISSN: 1424-8220 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology
Website: http://www.mdpi.com/journal/sensors

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