Study of matrix metalloproteinase activity in patients with autoimmune thyroiditis

Abstract

One of the most important pathogenetic mechanisms of autoimmune thyroiditis (AIT) is the violation of immunological tolerance and the development of the autoimmune process, the markers of which are various biologically active substances, in particular, matrix metalloproteinases (MMP) of the extracellular matrix (ECM). MMPs play a crucial role in the development of pathological processes in these diseases, contributing­ to matrix degradation due to imbalance between the activity of enzymes and their inhibitors. The aim of the work was to study the activity of key metalloproteinases and the level of α2-macroglobulin in patients with autoimmune thyroiditis. The diagnosis of AIT was established based on the study of data on anamnesis, thyroid status, the results of ultrasound of TG, and the presence of antibodies to the thyroid-stimulating hormone receptor (TSH) in blood plasma. Patients were enrolled in 2 groups: group 1 – 74 patients with a manifest form of the disease; group 2 – 96 patients with a subclinical form of the disease. The study of matrix metalloprotein activity in the examined patients showed a statistically significant (P = 0.015) increase in MMP-3 and MMP-7 activity in patients with AIT compared to the corresponding parameters in persons of the control group. Thus, levels of MMP-3 and 7 were in the group of patients, respectively 56 (51.0; 59.0) and 4.6 (4.3; 5.2) ng/ml, in control 23.0 (16.0; 26.0) and 3.6 (3.4; 4.1) ng/ml, respectively.

Keywords

• autoimmune thyroiditis
• extracellular matrix
• matrix metalloproteinases
• α2-macroglobulin