International Journal of Molecular Sciences (Jul 2024)

The Scavenging Activity of Coenzyme Q<sub>10</sub> Plus a Nutritional Complex on Human Retinal Pigment Epithelial Cells

  • Maria Hernandez,
  • Sergio Recalde,
  • Jaione Bezunartea,
  • Maite Moreno-Orduña,
  • Idoia Belza,
  • Ainara Chas-Prat,
  • Elena Perugini,
  • Alfredo Garcia-Layana,
  • Patricia Fernández-Robredo

DOI
https://doi.org/10.3390/ijms25158070
Journal volume & issue
Vol. 25, no. 15
p. 8070

Abstract

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Age-related macular degeneration (AMD) and diabetic retinopathy (DR) are common retinal diseases responsible for most blindness in working-age and elderly populations. Oxidative stress and mitochondrial dysfunction play roles in these pathogenesis, and new therapies counteracting these contributors could be of great interest. Some molecules, like coenzyme Q10 (CoQ10), are considered beneficial to maintain mitochondrial homeostasis and contribute to the prevention of cellular apoptosis. We investigated the impact of adding CoQ10 (Q) to a nutritional antioxidant complex (Nutrof Total®; N) on the mitochondrial status and apoptosis in an in vitro hydrogen peroxide (H2O2)-induced oxidative stress model in human retinal pigment epithelium (RPE) cells. H2O2 significantly increased 8-OHdG levels (p p p p p p p IL1β, SOD2, and CAT gene expression (p IL1β gene expression, TUNEL, TNFα, caspase-1, and JC-1 (p 2O2, and NQ showed a synergist effect in caspase-3 (p p p 10 supplementation is effective in restoring/preventing apoptosis and mitochondrial stress-related damage, suggesting that it could be a valid strategy in degenerative processes such as AMD or DR.

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