Frontiers in Pharmacology (Aug 2023)
β-hydroxybutyrate impairs bovine oocyte maturation via pyruvate dehydrogenase (PDH) associated energy metabolism abnormality
- Kai-Yan Zhang,
- Kai-Yan Zhang,
- Kai-Yan Zhang,
- Jing Guo,
- Jing Guo,
- Jing Guo,
- Cheng-Lin Zhan,
- Cheng-Lin Zhan,
- Cheng-Lin Zhan,
- Chong-Shan Yuan,
- Chong-Shan Yuan,
- Chong-Shan Yuan,
- Chang-Guo Min,
- Chang-Guo Min,
- Chang-Guo Min,
- Zhi-Qiang Li,
- Zhi-Qiang Li,
- Zhi-Qiang Li,
- Hong-Yu Liu,
- Hong-Yu Liu,
- Hong-Yu Liu,
- Jun Wang,
- Jun Wang,
- Jun Wang,
- Jing Zhao,
- Jing Zhao,
- Jing Zhao,
- Wen-Fa Lu,
- Wen-Fa Lu,
- Wen-Fa Lu,
- Xin Ma,
- Xin Ma,
- Xin Ma
Affiliations
- Kai-Yan Zhang
- Key Laboratory of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China
- Kai-Yan Zhang
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, Jilin, China
- Kai-Yan Zhang
- College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China
- Jing Guo
- Key Laboratory of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China
- Jing Guo
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, Jilin, China
- Jing Guo
- College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China
- Cheng-Lin Zhan
- Key Laboratory of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China
- Cheng-Lin Zhan
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, Jilin, China
- Cheng-Lin Zhan
- College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China
- Chong-Shan Yuan
- Key Laboratory of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China
- Chong-Shan Yuan
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, Jilin, China
- Chong-Shan Yuan
- College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China
- Chang-Guo Min
- Key Laboratory of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China
- Chang-Guo Min
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, Jilin, China
- Chang-Guo Min
- College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China
- Zhi-Qiang Li
- Key Laboratory of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China
- Zhi-Qiang Li
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, Jilin, China
- Zhi-Qiang Li
- College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China
- Hong-Yu Liu
- Key Laboratory of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China
- Hong-Yu Liu
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, Jilin, China
- Hong-Yu Liu
- College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China
- Jun Wang
- Key Laboratory of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China
- Jun Wang
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, Jilin, China
- Jun Wang
- College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China
- Jing Zhao
- Key Laboratory of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China
- Jing Zhao
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, Jilin, China
- Jing Zhao
- College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China
- Wen-Fa Lu
- Key Laboratory of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China
- Wen-Fa Lu
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, Jilin, China
- Wen-Fa Lu
- College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China
- Xin Ma
- Key Laboratory of the Animal Production, Product Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin, China
- Xin Ma
- Jilin Provincial International Joint Research Center of Animal Breeding and Reproduction Technology, Jilin Agricultural University, Changchun, Jilin, China
- Xin Ma
- College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin, China
- DOI
- https://doi.org/10.3389/fphar.2023.1243243
- Journal volume & issue
-
Vol. 14
Abstract
Background: Ketosis is one of the most frequent and costly metabolic disorders in high-producing dairy cows, and negatively associated with the health and reproductive performance of bovine. Ketosis is mainly caused by the accumulation of ketone body β-hydroxybutyric acid and its diagnosis is based on β-hydroxybutyrate (βHB) concentration in blood.Methods: In this study, we investigated the effects of βHB on bovine oocyte maturation in the concentration of subclinical (1.2 mM) βHB and clinical (3.6 mM).Results: The results showed βHB disrupted bovine oocyte maturation and development capacity. Further analysis showed that βHB induced oxidative stress and mitochondrial dysfunction, as indicated by the increased level of reactive oxygen species (ROS), disrupted mitochondrial structure and distribution, and depolarized membrane potential. Furthermore, oxidative stress triggered early apoptosis, as shown by the enhanced levels of Caspase-3 and Annexin-V. Moreover, 3.6 mM βHB induced the disruption of the pyruvate dehydrogenase (PDH) activity, showing with the decrease of the global acetylation modification and the increase of the abnormal spindle rate.Conclusion: Our study showed that βHB in subclinical/clinical concentration had toxic effects on mitochondrial function and PDH activity, which might affect energy metabolism and epigenetic modification of bovine oocytes and embryos.
Keywords
- β-hydroxybutyrate (βHB)
- PDH
- acetylation modification
- oocyte maturation
- bovine reproductive performance
