Sternbergia lutea, a new host of Narcissus late season yellows virus

Abstract

In autumn 2017, autumn daffodil plants with yellow-green stripes on the leaves were observed at a botanical garden in Budapest, Hungary. Indicator plants were inoculated, but symptoms did not develop. RT-PCR tests of the indicator plants were also negative for the viruses. Potyvirus specific ACP-ELISA and RT-PCR were carried out on the symptomatic S. lutea leaf samples. RT-PCR with universal potyvirus primers resulted in one, approx. 1700 base pair PCR product. Phylogenetic analysis of the nucleotide sequence of the coat protein demonstrated 98.78-99.51% identity with three Japanese isolates of Narcissus late season yellows virus. While unidentified potyvirus infection of autumn daffodil has been previously reported, sequence data have not been published. Therefore, this is the first report of Sternbergia lutea as a host of Narcissus late season yellows virus.

Keywords

• Potyvirus
• Hungary
• daffodil