Species differentiation of thermotolerant Campylobacters based on distinctive banding patterns obtained by multiplex PCR

Lucie VONDRÁKOVÁ; Sabina PURKRTOVÁ; Jarmila PAZLAROVÁ; Kateřina DEMNEROVÁ

doi:10.17221/267/2014-CJFS

Czech Journal of Food Sciences (Feb 2015)

Species differentiation of thermotolerant Campylobacters based on distinctive banding patterns obtained by multiplex PCR

Lucie VONDRÁKOVÁ,
Sabina PURKRTOVÁ,
Jarmila PAZLAROVÁ,
Kateřina DEMNEROVÁ

Affiliations

Lucie VONDRÁKOVÁ: Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Prague, Czech Republic
Sabina PURKRTOVÁ: Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Prague, Czech Republic
Jarmila PAZLAROVÁ: Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Prague, Czech Republic
Kateřina DEMNEROVÁ: Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Prague, Czech Republic

DOI: https://doi.org/10.17221/267/2014-CJFS
Journal volume & issue: Vol. 33, no. 1
pp. 27 – 31

Abstract

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The differentiation of thermotolerant Campylobacter spp. on the species level (C. jejuni, C. coli, C. lari, and C. upsalien-sis) was provided. Identification is based on different banding patterns obtained for individual species during simple multiplex PCR where regions within the 23S rRNA gene are amplified using newly designed specific forward primers.

Published in Czech Journal of Food Sciences

ISSN: 1212-1800 (Print); 1805-9317 (Online)
Publisher: Czech Academy of Agricultural Sciences
Country of publisher: Czechia
LCC subjects: Agriculture
Website: https://cjfs.agriculturejournals.cz/

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