Zhongguo youzhi (Jul 2022)

天然抗氧化剂对鳄鱼油体外抗氧化活性的影响Effects of natural antioxidant on in vitro antioxidant activity of crocodile oil

  • 傅丽君1,2 ，王海燕3 ，郑雅鸿1,2 ，杨磊1,2 ，李华亮4 ，丁玉梅4FU Lijun1,2 ,WANG Haiyan3 ,ZHENG Yahong1,2， YANG Lei1,2，LI Hualiang4，DING Yumei4

DOI
https://doi.org/10.19902/j.cnki.zgyz.1003-7969.210363
Journal volume & issue
Vol. 47, no. 7
pp. 116 – 120

Abstract

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为了筛选天然抗氧化剂和提高鳄鱼油体外抗氧化活性，采用Schaal烘箱法，比较3种常见的天然抗氧化剂茶多酚（TP)、维生素E和植酸对精制鳄鱼油的抗氧化效果，并优选出了天然抗氧化剂。在此基础上以优选抗氧化剂和VC为阳性对照，采用体外抗氧化法考察添加优选抗氧化剂的鳄鱼油对DPPH自由基、羟自由基（·OH）、超氧自由基（O-2·）清除能力，还原能力及Fe2+螯合能力。结果表明：3种天然抗氧化剂对鳄鱼油的抗氧化效果为TP＞维生素E＞植酸；鳄鱼油对DPPH自由基、·OH、O-2·清除能力,还原能力和Fe2+螯合能力均随其质量浓度的增加而升高；添加0.02%TP后鳄鱼油的体外抗氧化活性明显增强，添加TP的鳄鱼油对DPPH自由基、·OH和O-2·的IC50分别为0.874、0.064 mg/mL和0.96 mg/mL，均低于鳄鱼油的（5.448、0.684、4.53 mg/mL），而还原能力和Fe2+螯合能力均高于鳄鱼油的。综上，TP可有效提高鳄鱼油的体外抗氧化活性。 n order to screen natural antioxidant and improve the in vitro antioxidant activity of crocodile, the Schaal oven method was used to compare the antioxidant effects of three common natural antioxidants tea polyphenols (TP), VE and phytic acid on refined crocodile oil, and the antioxidant with stronger antioxidant capacity was selected. Then the DPPH radical, hydroxyl radical (·OH), superoxiden anion radical (O-2·) scavenging ability, reducing capacity and Fe2+ chelating capacity of crocodile oil added with selected antioxidant was evaluated by in vitro antioxidant method using added antioxidants and VC as positive control. The results showed that the antioxidant effect of TP was the strongest, followed by VE, and the antioxidant effect of phytic acid was the lowest.The scavenging ability of crocodile oil to DPPH free radical, ·OH,O-2· , and the reducing ability and Fe2+ chelating ability increased with the increase of crocodile oil mass concentration.The in vitro antioxidant capacity of crocodile oil was significantly enhanced by the addition of 0.02% TP. The IC50 of crocodile oil with TP against DPPH radicals, ·OH、O-2· were 0.874,0.064 mg/mL and 0.96 mg/mL, respectively, which were lower than those of crocodile oil (5.448, 0.684, 4.53 mg/mL), and the reducing ability and Fe2+ chelating ability were higher than those of crocodile oil. In summary, TP can effectively enhance the in vitro antioxidant activity of crocodile oil.

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