The Anti-Obesity Effects of Lemon Fermented Products in 3T3-L1 Preadipocytes and in a Rat Model with High-Calorie Diet-Induced Obesity

Chih-Chung Wu; Yu-Wen Huang; Chih-Yao Hou; Ya-Ting Chen; Cheng-Di Dong; Chiu-Wen Chen; Reeta-Rani Singhania; Jie-Yin Leang; Shu-Ling Hsieh

doi:10.3390/nu13082809

Nutrients (Aug 2021)

The Anti-Obesity Effects of Lemon Fermented Products in 3T3-L1 Preadipocytes and in a Rat Model with High-Calorie Diet-Induced Obesity

Chih-Chung Wu,
Yu-Wen Huang,
Chih-Yao Hou,
Ya-Ting Chen,
Cheng-Di Dong,
Chiu-Wen Chen,
Reeta-Rani Singhania,
Jie-Yin Leang,
Shu-Ling Hsieh

Affiliations

Chih-Chung Wu: Department of Food and Nutrition, Providence University, Taichung 43301, Taiwan
Yu-Wen Huang: Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan
Chih-Yao Hou: Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan
Ya-Ting Chen: Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan
Cheng-Di Dong: Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan
Chiu-Wen Chen: Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan
Reeta-Rani Singhania: Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan
Jie-Yin Leang: Department of Food Science, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan
Shu-Ling Hsieh: Department of Seafood Science, National Kaohsiung University of Science and Technology, Kaohsiung 81157, Taiwan

DOI: https://doi.org/10.3390/nu13082809
Journal volume & issue: Vol. 13, no. 8
p. 2809

Abstract

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Lemon (Citrus limon) has antioxidant, immunoregulatory, and blood lipid-lowering properties. This study aimed to determine the effect of the lemon fermented product (LFP) which is lemon fermented with Lactobacillus OPC1 to prevent obesity. The inhibition of lipid accumulation in 3T3-L1 adipocytes is examined using a Wistar rat model fed a high-fat diet to verify the anti-obesity efficacy and mechanism of LFP. Here, it was observed that LFP reduced cell proliferation and inhibited the lipid accumulation (8.3%) of 3T3-L1 adipocytes. Additionally, LFP reduced body weight (9.7%) and fat tissue weight (25.7%) of rats; reduced serum TG (17.0%), FFA (17.9%), glucose (29.3%) and ketone body (6.8%); and increased serum HDL-C (17.6%) and lipase activity (17.8%). LFP regulated the mRNA expression of genes related to lipid metabolism (PPARγ, C/EBPα, SREBP-1c, HSL, ATGL, FAS, and AMPK). Therefore, LFP reduces body weight and lipid accumulation by regulating the mRNA expression of genes related to lipid metabolism. Overall, our results implicate LFP as a potential dietary supplement for the prevention of obesity.

Published in Nutrients

ISSN: 2072-6643 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.mdpi.com/journal/nutrients

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