Frontiers in Physiology (May 2018)
Bushen Huoxue Attenuates Diabetes-Induced Cognitive Impairment by Improvement of Cerebral Microcirculation: Involvement of RhoA/ROCK/moesin and Src Signaling Pathways
- Yuan Li,
- Quan Li,
- Quan Li,
- Quan Li,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Li Yan,
- Li Yan,
- Li Yan,
- Bai-He Hu,
- Bai-He Hu,
- Bai-He Hu,
- Yu-Ying Liu,
- Yu-Ying Liu,
- Yu-Ying Liu,
- Lei Yang,
- Ping Huang,
- Ping Huang,
- Ping Huang,
- Shao-Yang Zhao,
- Chuan-She Wang,
- Chuan-She Wang,
- Chuan-She Wang,
- Chuan-She Wang,
- Jing-Yu Fan,
- Jing-Yu Fan,
- Jing-Yu Fan,
- Xue-Mei Wang,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han
Affiliations
- Yuan Li
- Integrated Laboratory of Traditional Chinese Medicine and Western Medicine, Peking University First Hospital, Beijing, China
- Quan Li
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Quan Li
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China
- Quan Li
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine, Beijing, China
- Chun-Shui Pan
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Chun-Shui Pan
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China
- Chun-Shui Pan
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine, Beijing, China
- Li Yan
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Li Yan
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China
- Li Yan
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine, Beijing, China
- Bai-He Hu
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Bai-He Hu
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China
- Bai-He Hu
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine, Beijing, China
- Yu-Ying Liu
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Yu-Ying Liu
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China
- Yu-Ying Liu
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine, Beijing, China
- Lei Yang
- Department of Anatomy, Peking University Health Science Center, Beijing, China
- Ping Huang
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Ping Huang
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China
- Ping Huang
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine, Beijing, China
- Shao-Yang Zhao
- Integrated Laboratory of Traditional Chinese Medicine and Western Medicine, Peking University First Hospital, Beijing, China
- Chuan-She Wang
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Chuan-She Wang
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China
- Chuan-She Wang
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine, Beijing, China
- Chuan-She Wang
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- Jing-Yu Fan
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Jing-Yu Fan
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China
- Jing-Yu Fan
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine, Beijing, China
- Xue-Mei Wang
- Integrated Laboratory of Traditional Chinese Medicine and Western Medicine, Peking University First Hospital, Beijing, China
- Jing-Yan Han
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Jing-Yan Han
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China
- Jing-Yan Han
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine, Beijing, China
- Jing-Yan Han
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- DOI
- https://doi.org/10.3389/fphys.2018.00527
- Journal volume & issue
-
Vol. 9
Abstract
Type 2 Diabetes mellitus (T2DM) is closely correlated with cognitive impairment and neurodegenerative disease. Bushen Huoxue (BSHX) is a compound Chinese medicine used clinically to treat diabetes-induced cognitive impairment. However, its underlying mechanisms remain unclear. In the present study, KKAy mice, a genetic model of type 2 diabetes with obesity and insulin resistant hyperglycemia, received a daily administration of BSHX for 12 weeks. Blood glucose was measured every 4 weeks. After 12 weeks, BSHX treatment significantly ameliorated the T2DM related insults, including the increased blood glucose, the impaired spatial memory, decreased cerebral blood flow (CBF), occurrence of albumin leakage, leukocyte adhesion and opening capillary rarefaction. Meanwhile, the downregulation of the tight junction proteins (TJ) claudin-5, occludin, zonula occluden-1 (ZO-1) and JAM-1 between endothelial cells, amyloid-β (Aβ) accumulation in hippocampus, increased AGEs and RAGE, and expression of RhoA/ROCK/moesin signaling pathway and phosphorylation of Src kinase in KKAy mice were significantly protected by BSHX treatment. These results indicate that the protective effect of BSHX on T2DM-induced cognitive impairment involves regulation of RhoA/ROCK1/moesin signaling pathway and phosphorylation of Src kinase.
Keywords
