Monoclonal antibody techniques. 50 years of development

Abstract

Monoclonal antibodies are widely used in all fields of biology and medicine. The emergence and development of the technology for their production revolutionized immunology and allowed the creation of not only new diagnostic methods, but also many effective drugs. The purpose of our review is to analyze and summarize relevant data concerning the technology of obtaining monoclonal antibodies. We have analyzed information from 70 modern literary sources devoted to various methods of obtaining them. In this review, we tried to cover the entire range of methods used to obtain monoclonal antibodies today.

Keywords

• monoclonal antibodies
• hybridomа technology
• recombinant dna
• recombinant antibodies
• phage display
• epstein–barr virus
• ngs
• crispr/cas9
• hepatitis c virus