Effects of glyphosate in common carp: Histopathological and immunohistochemical study

Abstract

We aimed in the current study to determine the LC50 of glyphosate in common carp fish (Cyprinus carpio L.) within 24 hours in addition to studying the pathological lesions of sublethal concentration of glyphosate (25 and 50% of LC50) in kidney and liver tissues. A total of 100 fish were used to determine the LC50 of glyphosate using the Trevan method (Curve protocol) within 24 hours, in addition to 75 fish used to study the histopathological lesions caused by 25 and 50% of LC50 in common carp after 5, 15 and 25 days of exposure. The current study showed that the LC50 of glyphosate in common carp was 26.151 mg/L within 24 hours, by this result the 25 and 50% of LC50 was 6.54 and 13.08 mg/L, respectively. The histopathological lesions revealed that both the kidney and liver showed degenerative and necrotic changes associated with infiltration of inflammatory cells and interstitial hemorrhages, also the liver sections showed hyperplasia of bile cuniculi and collagen fiber deposition within the portal area, generally, the histopathological lesions were more intensive and advanced in the group exposed to 50% of glyphosate LC50 more than that recorded in the group treated with 25% of glyphosate LC50. In conclusion, common carp fish (Cyprinus carpio L.) showed severe histopathological lesions after exposure to sublethal concentrations of glyphosate, these lesions are life-threatening and can cause high mortality in higher concentrations.

Keywords

• organophosphorus
• cyprinus carpio
• il-6
• il-12