A major and stable QTL for wheat spikelet number per spike validated in different genetic backgrounds

Pu-yang DING; Zi-qiang MO; Hua-ping TANG; Yang MU; Mei DENG; Qian-tao JIANG; Ya-xi LIU; Guang-deng CHEN; Guo-yue CHEN; Ji-rui WANG; Wei LI; Peng-fei QI; Yun-feng JIANG; Hou-yang KANG; Gui-jun YAN; Yu-ming WEI; You-liang ZHENG; Xiu-jin LAN; Jian MA

Journal of Integrative Agriculture (Jun 2022)

A major and stable QTL for wheat spikelet number per spike validated in different genetic backgrounds

Pu-yang DING,
Zi-qiang MO,
Hua-ping TANG,
Yang MU,
Mei DENG,
Qian-tao JIANG,
Ya-xi LIU,
Guang-deng CHEN,
Guo-yue CHEN,
Ji-rui WANG,
Wei LI,
Peng-fei QI,
Yun-feng JIANG,
Hou-yang KANG,
Gui-jun YAN,
Yu-ming WEI,
You-liang ZHENG,
Xiu-jin LAN,
Jian MA

Affiliations

Pu-yang DING: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Zi-qiang MO: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Hua-ping TANG: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Yang MU: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Mei DENG: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Qian-tao JIANG: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Ya-xi LIU: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Guang-deng CHEN: College of Resources, Sichuan Agricultural University, Chengdu 611130, P.R.China
Guo-yue CHEN: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Ji-rui WANG: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Wei LI: College of Agronomy, Sichuan Agricultural University, Chengdu 611130, P.R.China
Peng-fei QI: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Yun-feng JIANG: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Hou-yang KANG: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Gui-jun YAN: UWA School of Agriculture and Environment and The UWA Institute of Agriculture, Faculty of Science, University of Western Australia, Crawley 6009, Australia
Yu-ming WEI: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
You-liang ZHENG: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Xiu-jin LAN: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China; Correspondence LAN Xiu-jin
Jian MA: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China; Correspondence MA Jian, Tel: +86-28-86293115, Fax: +86-28-82650350

Journal volume & issue: Vol. 21, no. 6
pp. 1551 – 1562

Abstract

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The spikelet number per spike (SNS) contributes greatly to grain yield in wheat. Identifying various genes that control wheat SNS is vital for yield improvement. This study used a recombinant inbred line population genotyped by the Wheat55K single-nucleotide polymorphism array to identify two major and stably expressed quantitative trait loci (QTLs) for SNS. One of them (QSns.sau-2SY-2D.1) was reported previously, while the other (QSns.sau-2SY-7A) was newly detected and further analyzed in this study. QSns.sau-2SY-7A had a high LOD value ranging from 4.46 to 16.00 and explained 10.21–40.78% of the phenotypic variances. QSns.sau-2SY-7A was flanked by the markers AX-110518554 and AX-110094527 in a 4.75-cM interval on chromosome arm 7AL. The contributions and interactions of both major QTLs were further analyzed and discussed. The effect of QSns.sau-2SY-7A was successfully validated by developing a tightly linked kompetitive allele specific PCR marker in an F2:3 population and a panel of 101 high-generation breeding wheat lines. Furthermore, several genes including the previously reported WHEAT ORTHOLOG OF APO1 (WAPO1), an ortholog of the rice gene ABERRANT PANICLE ORGANIZATION 1 (APO1) related to SNS, were predicted in the interval of QSns.sau-2SY-7A. In summary, these results revealed the genetic basis of the multi-spikelet genotype of wheat line 20828 and will facilitate subsequent fine mapping and breeding utilization of the major QTLs.

Published in Journal of Integrative Agriculture

ISSN: 2095-3119 (Print); 2352-3425 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.journals.elsevier.com/journal-of-integrative-agriculture

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