De novo assembly and comparison of the ovarian transcriptomes of the common Chinese cuttlefish (Sepiella japonica) with different gonadal development

Zhenming Lü; Wan Liu; Liqin Liu; Huilai Shi; Hongling Ping; Tianming Wang; Changfeng Chi; Changwen Wu; Ching-Hung Chen; Kang-Ning Shen; Chung-Der Hsiao

Genomics Data (Mar 2016)

De novo assembly and comparison of the ovarian transcriptomes of the common Chinese cuttlefish (Sepiella japonica) with different gonadal development

Zhenming Lü,
Wan Liu,
Liqin Liu,
Huilai Shi,
Hongling Ping,
Tianming Wang,
Changfeng Chi,
Changwen Wu,
Ching-Hung Chen,
Kang-Ning Shen,
Chung-Der Hsiao

Affiliations

Zhenming Lü: National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan 316004, China
Wan Liu: National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan 316004, China
Liqin Liu: National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan 316004, China
Huilai Shi: Marine Fisheries Research Institute of Zhejiang, Zhoushan 316021, China
Hongling Ping: Marine Fisheries Research Institute of Zhejiang, Zhoushan 316021, China
Tianming Wang: National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan 316004, China
Changfeng Chi: National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan 316004, China
Changwen Wu: National Engineering Research Center for Facilitated Marine Aquaculture, Marine Science College, Zhejiang Ocean University, Zhoushan 316004, China
Ching-Hung Chen: WeThink Biotech Inc., Taoyuan, Taiwan
Kang-Ning Shen: Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 20224, Taiwan; Correspondence to: K.-N. Shen, Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, 20224, Taiwan.
Chung-Der Hsiao: Department of Bioscience Technology, Chung Yuan Christian University, Chung-Li 32023, Taiwan; Correspondence to: C.-D. Hsiao, 200 Chung-Pei Rd., Department of Bioscience Technology, Chung Yuan Christian University, 32023 Chung-Li, Taiwan.

Journal volume & issue: Vol. 7
pp. 155 – 158

Abstract

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The common Chinese cuttlefish (Sepiella japonica) has been considered one of the most economically important marine Cephalopod species in East Asia and seed breeding technology has been established for massive aquaculture and stock enhancement. In the present study, we used Illumina HiSeq2000 to sequence, assemble and annotate the transcriptome of the ovary tissues of S. japonica for the first time. A total of 53,116,650 and 53,446,640 reads were obtained from the immature and matured ovaries, respectively (NCBI SRA database SRX1409472 and SRX1409473), and 70,039 contigs (N50 = 1443 bp) were obtained after de novo assembling with Trinity software. Digital gene expression analysis reveals 47,288 contigs show differential expression profile and 793 contigs are highly expressed in the immature ovary, while 38 contigs are highly expressed in the mature ovary with FPKM >100. We hope that the ovarian transcriptome and those stage-enriched transcripts of S. japonica can provide some insight into the understanding of genome-wide transcriptome profile of cuttlefish gonad tissue and give useful information in cuttlefish gonad development. Keywords: Cuttlefish, Gonad development, Transcriptome

Published in Genomics Data

ISSN: 2213-5960 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Biology (General): Genetics
Website: http://www.journals.elsevier.com/genomics-data/

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