A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

Qixin Chen; Hongbao Fang; Xintian Shao; Zhiqi Tian; Shanshan Geng; Yuming Zhang; Huaxun Fan; Pan Xiang; Jie Zhang; Xiaohe Tian; Kai Zhang; Weijiang He; Zijian Guo; Jiajie Diao

doi:10.1038/s41467-020-20067-6

Nature Communications (Dec 2020)

A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells

Qixin Chen,
Hongbao Fang,
Xintian Shao,
Zhiqi Tian,
Shanshan Geng,
Yuming Zhang,
Huaxun Fan,
Pan Xiang,
Jie Zhang,
Xiaohe Tian,
Kai Zhang,
Weijiang He,
Zijian Guo,
Jiajie Diao

Affiliations

Qixin Chen: State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University
Hongbao Fang: State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University
Xintian Shao: Department of Cancer Biology, University of Cincinnati College of Medicine
Zhiqi Tian: Department of Cancer Biology, University of Cincinnati College of Medicine
Shanshan Geng: State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University
Yuming Zhang: State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University
Huaxun Fan: Department of Biochemistry, University of Illinois at Urbana-Champaign
Pan Xiang: Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Anhui University
Jie Zhang: Advanced Medical Research Institute/Translational Medicine Core Facility of Advanced Medical Research Institute, Shandong University
Xiaohe Tian: Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Anhui University
Kai Zhang: Department of Biochemistry, University of Illinois at Urbana-Champaign
Weijiang He: State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University
Zijian Guo: State Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University
Jiajie Diao: Department of Cancer Biology, University of Cincinnati College of Medicine

DOI: https://doi.org/10.1038/s41467-020-20067-6
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

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Dynamic labeling and tracking of organelle–organelle contacts is essential to understand the formation and function of these interactions. Here the authors present a small molecule probe, Coupa, that labels mitochondria and lysosomes with blue and red fluorescence, respectively.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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