Identification of nitrated proteins in Alzheimer's disease brain using a redox proteomics approach

Rukhsana Sultana; H. Fai Poon; Jian Cai; William M. Pierce; Michael Merchant; Jon B. Klein; William R. Markesbery; D. Allan Butterfield

Neurobiology of Disease (Apr 2006)

Identification of nitrated proteins in Alzheimer's disease brain using a redox proteomics approach

Rukhsana Sultana,
H. Fai Poon,
Jian Cai,
William M. Pierce,
Michael Merchant,
Jon B. Klein,
William R. Markesbery,
D. Allan Butterfield

Affiliations

Rukhsana Sultana: Department of Chemistry, University of Kentucky, Lexington, KY 40506, USA; Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY 40506, USA; Center of Membrane Sciences, University of Kentucky, Lexington, KY 40506, USA
H. Fai Poon: Department of Chemistry, University of Kentucky, Lexington, KY 40506, USA; Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY 40506, USA; Center of Membrane Sciences, University of Kentucky, Lexington, KY 40506, USA
Jian Cai: Department of Pharmacology, University of Louisville, School of Medicine and VAMC, Louisville, KY 40202, USA
William M. Pierce: Department of Pharmacology, University of Louisville, School of Medicine and VAMC, Louisville, KY 40202, USA
Michael Merchant: Core Proteomics Laboratory, University of Louisville, Louisville, KY 40208, USA
Jon B. Klein: Core Proteomics Laboratory, University of Louisville, Louisville, KY 40208, USA
William R. Markesbery: Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY 40506, USA; Department of Neurology and Pathology, University of Kentucky, Lexington, KY 40536, USA
D. Allan Butterfield: Department of Chemistry, University of Kentucky, Lexington, KY 40506, USA; Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY 40506, USA; Center of Membrane Sciences, University of Kentucky, Lexington, KY 40506, USA; Corresponding author. Department of Chemistry, Center of Membrane Sciences, and Sanders-Brown Center on Aging, University of Kentucky, Lexington, KY 40506, USA. Fax: +1 859 257 5876.

Journal volume & issue: Vol. 22, no. 1
pp. 76 – 87

Abstract

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Nitric oxide (NO) has been implicated in the pathophysiology of a number of neurodegenerative diseases including Alzheimer's disease (AD). In the present study, using a proteomics approach, we identified enolase, glyceraldehyde-3-phosphate dehydrogenase, ATP synthase alpha chain, carbonic anhydrase-II, and voltage-dependent anion channel—protein as the targets of nitration in AD hippocampus, a region that shows a extensive deposition of amyloid β-peptide, compared with the age-matched control brains. Immunoprecipitation and Western blotting techniques were used to validate the correct identification of these proteins. Our results are discussed in context of the role of oxidative stress as one of the important mechanisms of neurodegeneration in AD.

Published in Neurobiology of Disease

ISSN: 0969-9961 (Print); 1095-953X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.journals.elsevier.com/neurobiology-of-disease

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