Whole-cell imaging of plasma membrane receptors by 3D lattice light-sheet dSTORM

Felix Wäldchen; Jan Schlegel; Ralph Götz; Michael Luciano; Martin Schnermann; Sören Doose; Markus Sauer

doi:10.1038/s41467-020-14731-0

Nature Communications (Feb 2020)

Whole-cell imaging of plasma membrane receptors by 3D lattice light-sheet dSTORM

Felix Wäldchen,
Jan Schlegel,
Ralph Götz,
Michael Luciano,
Martin Schnermann,
Sören Doose,
Markus Sauer

Affiliations

Felix Wäldchen: Department of Biotechnology and Biophysics, Biocenter, University of Würzburg
Jan Schlegel: Department of Biotechnology and Biophysics, Biocenter, University of Würzburg
Ralph Götz: Department of Biotechnology and Biophysics, Biocenter, University of Würzburg
Michael Luciano: Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute
Martin Schnermann: Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute
Sören Doose: Department of Biotechnology and Biophysics, Biocenter, University of Würzburg
Markus Sauer: Department of Biotechnology and Biophysics, Biocenter, University of Würzburg

DOI: https://doi.org/10.1038/s41467-020-14731-0
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 6

Abstract

Read online

The organisation of plasma membrane receptors is important for their function. Here the authors combine lattice light-sheet microscopy with 3D single-molecule localisation microscopy (dSTORM) and single-particle tracking to study the distribution and motility of several surface receptors.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal