Improved immunoassay sensitivity and specificity using single-molecule colocalization

Amani A. Hariri; Sharon S. Newman; Steven Tan; Dan Mamerow; Alexandra M. Adams; Nicolò Maganzini; Brian L. Zhong; Michael Eisenstein; Alexander R. Dunn; H. Tom Soh

doi:10.1038/s41467-022-32796-x

Nature Communications (Sep 2022)

Improved immunoassay sensitivity and specificity using single-molecule colocalization

Amani A. Hariri,
Sharon S. Newman,
Steven Tan,
Dan Mamerow,
Alexandra M. Adams,
Nicolò Maganzini,
Brian L. Zhong,
Michael Eisenstein,
Alexander R. Dunn,
H. Tom Soh

Affiliations

Amani A. Hariri: Department of Radiology, Stanford University
Sharon S. Newman: Department of Bioengineering, Stanford University
Steven Tan: Department of Chemical Engineering, Stanford University
Dan Mamerow: Department of Radiology, Stanford University
Alexandra M. Adams: Department of Chemical Engineering, Stanford University
Nicolò Maganzini: Department of Electrical Engineering, Stanford University
Brian L. Zhong: Department of Chemical Engineering, Stanford University
Michael Eisenstein: Department of Radiology, Stanford University
Alexander R. Dunn: Department of Chemical Engineering, Stanford University
H. Tom Soh: Department of Radiology, Stanford University

DOI: https://doi.org/10.1038/s41467-022-32796-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 11

Abstract

Read online

A major challenge of enzyme-linked immunosorbent assays is discriminating true signal from non-specific binding. Here the authors present a Single-Molecule Colocalization Assay (SiMCA) which eliminates such effects, enabling reproducible detection of picomolar protein concentrations.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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