Generation of integration-free human induced pluripotent stem cells from a patient with sporadic Parkinson’s disease

Yukyeong Lee; Dahee Jeong; Seokbeom Ham; Jaeseung Son; Kinarm Ko

Stem Cell Research (Jun 2024)

Generation of integration-free human induced pluripotent stem cells from a patient with sporadic Parkinson’s disease

Yukyeong Lee,
Dahee Jeong,
Seokbeom Ham,
Jaeseung Son,
Kinarm Ko

Affiliations

Yukyeong Lee: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul 05029, Republic of Korea
Dahee Jeong: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul 05029, Republic of Korea
Seokbeom Ham: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul 05029, Republic of Korea
Jaeseung Son: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul 05029, Republic of Korea
Kinarm Ko: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul 05029, Republic of Korea; Center for Stem Cell Research, Institute of Advanced Biomedical Science, Konkuk University, Seoul 05029, Republic of Korea; Corresponding author at: Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul 05029, Republic of Korea.

Journal volume & issue: Vol. 77
p. 103416

Abstract

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A human induced pluripotent stem cell (iPSC) line (KKUi002-A) was generated from a skin fibroblast of a 57-years-old (at sampling) male patient diagnosed with a sporadic Parkinson’s disease (PD). A non-integration system was used to reprogram fibroblasts into iPSCs by an episomal vector (OCT4/p53, SOX2/KLF4, L-MYC/LIN28). The KKUi002-A iPSCs displayed typical iPSC morphology, expressed pluripotency markers, differentiated into derivatives of three germ layers, and had a normal karyotype. These PD-derived iPSCs can be used to understand the mechanism underlying PD pathogenesis.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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