Arquivos Brasileiros de Cardiologia (Jul 2015)

p.Q192R SNP of <italic>PON1</italic> seems not to be Associated with Carotid Atherosclerosis Risk Factors in an Asymptomatic and Normolipidemic Brazilian Population Sample

  • Daniel Zanetti Scherrer,
  • Vanessa Helena de Souza Zago,
  • Isabela Calanca Vieira,
  • Eliane Soler Parra,
  • Natália Baratella Panzoldo,
  • Fernanda Alexandre,
  • Rodrigo Secolin,
  • Jamal Baracat,
  • Eder Carlos Rocha Quintão,
  • Eliana Cotta de Faria

DOI
https://doi.org/10.5935/abc.20150053
Journal volume & issue
Vol. 105, no. 1
pp. 45 – 52

Abstract

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Background:Evidences suggest that paraoxonase 1 (PON1) confers important antioxidant and anti-inflammatory properties when associated with high-density lipoprotein (HDL).Objective:To investigate the relationships between p.Q192R SNP of PON1, biochemical parameters and carotid atherosclerosis in an asymptomatic, normolipidemic Brazilian population sample.Methods:We studied 584 volunteers (females n = 326, males n = 258; 19-75 years of age). Total genomic DNA was extracted and SNP was detected in the TaqMan® SNP OpenArray® genotyping platform (Applied Biosystems, Foster City, CA). Plasma lipoproteins and apolipoproteins were determined and PON1 activity was measured using paraoxon as a substrate. High-resolution β-mode ultrasonography was used to measure cIMT and the presence of carotid atherosclerotic plaques in a subgroup of individuals (n = 317).Results:The presence of p.192Q was associated with a significant increase in PON1 activity (RR = 12.30 (11.38); RQ = 46.96 (22.35); QQ = 85.35 (24.83) μmol/min; p Conclusion:In low-risk individuals, the presence of the p.192Q variant of PON1 is associated with a beneficial plasma lipid profile but not with carotid atherosclerosis.

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