Current Issues in Molecular Biology (Oct 2023)

Neuroprotective Effects of Water Extract from Brown Algae <i>Petalonia binghamiae</i> in an Experimental Model of Focal Cerebral Ischemia In Vitro and In Vivo

  • Sun Ho Eom,
  • Geum-Lan Hong,
  • Hyun Bae Kang,
  • Nam-Seob Lee,
  • Do Kyung Kim,
  • Young Gil Jeong,
  • Chun-Sung Kim,
  • Yung Choon Yoo,
  • Bong Ho Lee,
  • Ju-Young Jung,
  • Dong-Sub Kim,
  • Seung Yun Han

DOI
https://doi.org/10.3390/cimb45100531
Journal volume & issue
Vol. 45, no. 10
pp. 8427 – 8443

Abstract

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Focal cerebral ischemia (fCI) can result in brain injury and sensorimotor deficits. Brown algae are currently garnering scientific attention as potential therapeutic candidates for fCI. This study investigated the therapeutic effects of the hot water extract of Petalonia binghamiae (wPB), a brown alga, in in vitro and in vivo models of fCI. The neuroprotective efficacy of wPB was evaluated in an in vitro excitotoxicity model established using HT-22 cells challenged with glutamate. Afterward, C57/BL6 mice were administered wPB for 7 days (10 or 100 mg/kg, intragastric) and subjected to middle cerebral artery occlusion and reperfusion (MCAO/R) operation, which was used as an in vivo fCI model. wPB co-incubation significantly inhibited cell death, oxidative stress, and apoptosis, as well as stimulated the expression of heme oxygenase-1 (HO-1), an antioxidant enzyme, and the nuclear translocation of its upstream regulator, nuclear factor erythroid 2-related factor 2 (Nrf2) in HT-22 cells challenged with glutamate-induced excitotoxicity. Pretreatment with either dose of wPB significantly attenuated infarction volume, neuronal death, and sensorimotor deficits in an in vivo fCI model. Furthermore, the attenuation of oxidative stress and apoptosis in the ischemic lesion accompanied the wPB-associated protection. This study suggests that wPB can counteract fCI via an antioxidative effect, upregulating the Nrf2/HO-1 pathway.

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