Generation and characterisation of a human induced pluripotent stem cell line, NIMHi007-A, from peripheral blood mononuclear cells derived from an adult healthy female

Madhura Milind Nimonkar; Gautham Arunachal; Suravi Sasmita Dash; Swapna Kannothum Kandy; Kenchaiah Raghavendra; Ghati K Chetan; Bhupesh Mehta; Yogananda S Markandeya

Stem Cell Research (Aug 2023)

Generation and characterisation of a human induced pluripotent stem cell line, NIMHi007-A, from peripheral blood mononuclear cells derived from an adult healthy female

Madhura Milind Nimonkar,
Gautham Arunachal,
Suravi Sasmita Dash,
Swapna Kannothum Kandy,
Kenchaiah Raghavendra,
Ghati K Chetan,
Bhupesh Mehta,
Yogananda S Markandeya

Affiliations

Madhura Milind Nimonkar: Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India
Gautham Arunachal: Department of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru, Karnataka, India
Suravi Sasmita Dash: Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India
Swapna Kannothum Kandy: Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India
Kenchaiah Raghavendra: Department of Neurology, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India
Ghati K Chetan: Department of Human Genetics, National Institute of Mental Health and Neuro Sciences (NIMHANS), Bengaluru, Karnataka, India
Bhupesh Mehta: Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India
Yogananda S Markandeya: Department of Biophysics, National Institute of Mental Health and Neurosciences (NIMHANS), Bengaluru, India; Corresponding author.

Journal volume & issue: Vol. 70
p. 103130

Abstract

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We report the generation and characterisation of a human induced pluripotent stem cell (iPSC) line, NIMHi007-A, derived from peripheral blood mononuclear cells (PBMCs) of a healthy female adult individual. PBMCs were reprogrammed using the non-integrating Sendai virus consisting of Yamanaka reprogramming factors- SOX2, cMYC, KLF4, and OCT4. The iPSCs displayed a normal karyotype, express pluripotency markers, and could generate into three germ layers, endoderm, mesoderm, and ectoderm, in-vitro. This iPSC line, NIMHi007-A, can be used as a healthy control for various in-vitro disease models and study their underlying pathophysiological mechanisms.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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