14-3-3  Protein is Detectable in Blood Serum and Fetlock Joint Synovial Fluid of High Performance Horses

Abstract

Objective: To investigate whether 14-3-3 eta protein is detectable in synovial fluid and blood serum of high performance horses and to study whether any significant correlation between this and matrix metalloproteinase 1 and 3 in horse synovial fluid. Design: Clinical study. Animals: Eleven standard breed horses (seven high performances and four controls). Procedures: Blood serum and fetlock synovial fluid of seven high performance and four control horses were subjected to western blot for detection of 14-3-3 eta, gama, matrix metalloproteinase 1 and 3 proteins. Results: High level of 14-3-3 eta protein was detected in synovial fluid of high performance horses compare to those of control (P Conclusions and Clinical Relevance: 14-3-3 eta isoform can be readily detected in synovial fluid and blood serum of high performance horses and presence of this protein has significant effect on the levels of matrix metalloproteinase 1 and 3 in synovial fluid. As the high performance horses are always prone to joint diseases, this protein might be use as an early diagnostic tool in some joint inflammatory conditions.

Keywords

• horse
• 14-3-3 eta
• matrix metalloproteinase
• Joint
• arthritis