Investigating the Electric Field Lysis of Exosomes Immobilized on the Screen-Printed Electrode and Electrochemical Sensing of the Lysed-Exosome-Derived Protein

Krishna Thej Pammi Guru; Nusrat Praween; Palash Kumar Basu

doi:10.3390/bios13030323

Biosensors (Feb 2023)

Investigating the Electric Field Lysis of Exosomes Immobilized on the Screen-Printed Electrode and Electrochemical Sensing of the Lysed-Exosome-Derived Protein

Krishna Thej Pammi Guru,
Nusrat Praween,
Palash Kumar Basu

Affiliations

Krishna Thej Pammi Guru: Department of Avionics, Indian Institute of Space Science and Technology, Thiruvanathapuram 695547, India
Nusrat Praween: Department of Avionics, Indian Institute of Space Science and Technology, Thiruvanathapuram 695547, India
Palash Kumar Basu: Department of Avionics, Indian Institute of Space Science and Technology, Thiruvanathapuram 695547, India

DOI: https://doi.org/10.3390/bios13030323
Journal volume & issue: Vol. 13, no. 3
p. 323

Abstract

Read online

It is important to isolate exosomes (8 exosomes/mL were isolated from serum. With the limit of detection of 10 pg, the designed cell showed a linear detection of HER2 from 0.1 ng to 1 µg. It was observed from the results that the electric field lysis of exosomes not only plays a significant role in releasing the cargo protein but also improves the sensing of surface proteins associated with exosomes.

Published in Biosensors

ISSN: 2079-6374 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.mdpi.com/journal/biosensors

About the journal

Abstract

Keywords