Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions

Oksana V. Tkachenko; Nina V. Evseeva; Kristina Y. Kargapolova; Alena Y. Denisova; Natalia N. Pozdnyakova; Artem A. Kulikov; Gennady L. Burygin

doi:10.3390/microorganisms11071866

Microorganisms (Jul 2023)

Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions

Oksana V. Tkachenko,
Nina V. Evseeva,
Kristina Y. Kargapolova,
Alena Y. Denisova,
Natalia N. Pozdnyakova,
Artem A. Kulikov,
Gennady L. Burygin

Affiliations

Oksana V. Tkachenko: Department of Plant Breeding, Selection, and Genetics, Faculty of Agronomy, Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov, 410012 Saratov, Russia
Nina V. Evseeva: Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 410049 Saratov, Russia
Kristina Y. Kargapolova: Department of Plant Breeding, Selection, and Genetics, Faculty of Agronomy, Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov, 410012 Saratov, Russia
Alena Y. Denisova: Department of Plant Breeding, Selection, and Genetics, Faculty of Agronomy, Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov, 410012 Saratov, Russia
Natalia N. Pozdnyakova: Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), 410049 Saratov, Russia
Artem A. Kulikov: Department of Plant Breeding, Selection, and Genetics, Faculty of Agronomy, Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov, 410012 Saratov, Russia
Gennady L. Burygin: Department of Plant Breeding, Selection, and Genetics, Faculty of Agronomy, Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov, 410012 Saratov, Russia

DOI: https://doi.org/10.3390/microorganisms11071866
Journal volume & issue: Vol. 11, no. 7
p. 1866

Abstract

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Adaptation ex vitro is strongly stressful for microplants. Plant-growth-promoting rhizobacteria (PGPR) help to increase the adaptation potential of microplants transplanted from test tubes into the natural environment. We investigated the mechanisms of antioxidant protection of PGPR-inoculated potato microclones adapting to ex vitro growth in an aeroponic system. Potato (Solanum tuberosum L. cv. Nevsky) microplants were inoculated in vitro with the bacteria Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2. On days 1 and 7 of plant growth ex vitro, catalase and peroxidase activities in the leaves of inoculated plants were 1.5-fold higher than they were in non-inoculated plants. The activity of ascorbate peroxidase was reduced in both in vitro and ex vitro treatments, and this reduction was accompanied by a decrease in the leaf content of hydrogen peroxide and malondialdehyde. As a result, inoculation contributed to the regulation of the plant pro/antioxidant system, lowering the oxidative stress and leading to better plant survival ex vitro. This was evidenced by the higher values of measured morphological and physiological variables of the inoculated plants, as compared with the values in the control treatment. Thus, we have shown some PGPR-mediated mechanisms of potato plant protection from adverse environmental factors under aeroponic conditions.

Published in Microorganisms

ISSN: 2076-2607 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General)
Website: https://www.mdpi.com/journal/microorganisms

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