OncoTargets and Therapy (Dec 2020)
Overexpression of LncRNA SNHG1 Were Suitable for Oncolytic Adenoviruse H101 Therapy in Oral Squamous-Cell Carcinoma
Abstract
Xin Wang, 1,* Song Yang, 1,* Xuechao Lv, 2 Lina Wang, 3 Chunmei Li 4 1Department of Plastic Maxillofacial Surgery, Heilongjiang Provincial Hospital, Harbin 150036, Heilongjiang, People’s Republic of China; 2Department of Pediatric Dentistry, School of Stomatology, Harbin Medical University, Harbin 150001, Heilongjiang, People’s Republic of China; 3Department of Oral and Maxillofacial Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang, People’s Republic of China; 4Department of Outpatient Stomatology, Heilongjiang Provincial Hospital, Harbin 150036, Heilongjiang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chunmei LiDepartment of Outpatient Stomatology, Heilongjiang Provincial Hospital, Harbin 150036, Heilongjiang, People’s Republic of ChinaTel +86 451-8713-1023Email [email protected]: As the most prevalent type of head and neck cancer, oral squamous-cell carcinoma (OSCC) accounts for nearly 90% of all oral cancer cases. Despite great progress having been made in the diagnosis and treatment of OSCC recently, the survival rate of OSCC patients has not risen remarkably. Chemotherapy is commonly used for OSCC treatment; however, the emergence of chemoresistance limits its long-term curative effect. Therefore, identifying effective biomarkers and molecular mechanisms is essential to the development of therapeutic strategies for OSCC.Methods: qRT-PCR assays were performed to detect SNHG1 expression in OSCC tissue and cells, and CCK8 assays and animal experiments used to examine cell proliferation. In addition, CCK8 assays were used to detect IC 50 values of cisplatin, 5Fu, Dox, and oncolytic adenovirus H101.Results: We found that SNHG1 was overexpressed in OSCC tissue and cells and was associated with OSCC progression. In addition, knockdown of SNHG1 suppressed cell proliferation in vitro and in vivo. Importantly, we found that oncolytic adenovirus H101 showed better antitumor effects in OSCC with high SNHG1 expression, and chemotherapy showed worse anti-tumor effects in OSCC with high SNHG1 expression.Conclusion: SNHG1 can act as a diagnostic biomarker for OSCC, and may be a biomarker for treatment options.Keywords: SNHG1, H101, OSCC
