Frontiers in Plant Science (Jul 2024)

Enhancing Pseudomonas syringae pv. Actinidiae sensitivity in kiwifruit by repressing the NBS-LRR genes through miRNA-215-3p and miRNA-29-3p identification

  • Chengyao Jiang,
  • Xiaoying Zhang,
  • Jiahui Rao,
  • Shu Luo,
  • Liang Luo,
  • Wei Lu,
  • Mengyao Li,
  • Shumei Zhao,
  • Dan Ren,
  • Jiaming Liu,
  • Yu Song,
  • Yangxia Zheng,
  • Yin-Biao Sun

DOI
https://doi.org/10.3389/fpls.2024.1403869
Journal volume & issue
Vol. 15

Abstract

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Kiwifruit bacterial canker, caused by Pseudomonas syringae pv. actinidiae (PSA), poses a grave threat to the global kiwifruit industry. In this study, we examined the role of microRNAs (miRNAs) in kiwifruit’s response to PSA. Kiwifruit seedlings subjected to PSA treatment showed significant changes in both miRNA and gene expression compared to the control group. We identified 364 differentially expressed miRNAs (DEMs) and 7170 differentially expressed genes (DEGs). Further analysis revealed 180 miRNAs negatively regulating 641 mRNAs. Notably, two miRNAs from the miRNA482 family, miRNA-215-3p and miRNA-29-3p, were found to increase kiwifruit’s sensitivity to PSA when overexpressed. These miRNAs were linked to the regulation of NBS-LRR target genes, shedding light on their role in kiwifruit’s defence against PSA. This study offers insights into the miRNA482-NBS-LRR network as a crucial component in enhancing kiwifruit bioresistance to PSA infestation and provides promising candidate genes for further research.

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