Nature Communications (Oct 2022)

Spatial snapshots of amyloid precursor protein intramembrane processing via early endosome proteomics

  • Hankum Park,
  • Frances V. Hundley,
  • Qing Yu,
  • Katherine A. Overmyer,
  • Dain R. Brademan,
  • Lia Serrano,
  • Joao A. Paulo,
  • Julia C. Paoli,
  • Sharan Swarup,
  • Joshua J. Coon,
  • Steven P. Gygi,
  • J. Wade Harper

DOI
https://doi.org/10.1038/s41467-022-33881-x
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 21

Abstract

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Methods to assess organellar content are important. Here, Park et al develop a method for rapid isolation of early/sorting endosomes and demonstrate the application of the approach for analysis of endosomal proteomes and lipidomes, and for analysis of APP processing to Aβ via β and γ-Secretases.