Nature Communications (Mar 2019)
Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design
- Xiaoyun Lu,
- Yuwan Liu,
- Yiqun Yang,
- Shanshan Wang,
- Qian Wang,
- Xiya Wang,
- Zhihui Yan,
- Jian Cheng,
- Cui Liu,
- Xue Yang,
- Hao Luo,
- Sheng Yang,
- Junran Gou,
- Luzhen Ye,
- Lina Lu,
- Zhidan Zhang,
- Yu Guo,
- Yan Nie,
- Jianping Lin,
- Sheng Li,
- Chaoguang Tian,
- Tao Cai,
- Bingzhao Zhuo,
- Hongwu Ma,
- Wen Wang,
- Yanhe Ma,
- Yongjun Liu,
- Yin Li,
- Huifeng Jiang
Affiliations
- Xiaoyun Lu
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Yuwan Liu
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Yiqun Yang
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Shanshan Wang
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Qian Wang
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Xiya Wang
- School of Chemistry and Chemical Engineering, Shandong University
- Zhihui Yan
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Jian Cheng
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Cui Liu
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Xue Yang
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Hao Luo
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Sheng Yang
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Junran Gou
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Luzhen Ye
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Lina Lu
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Zhidan Zhang
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Yu Guo
- University of Chinese Academy of Sciences
- Yan Nie
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Jianping Lin
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Sheng Li
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Chaoguang Tian
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Tao Cai
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Bingzhao Zhuo
- Center for Ecological and Environmental Sciences, Northwestern Polytechnical University
- Hongwu Ma
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Wen Wang
- Center for Ecological and Environmental Sciences, Northwestern Polytechnical University
- Yanhe Ma
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- Yongjun Liu
- School of Chemistry and Chemical Engineering, Shandong University
- Yin Li
- Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences
- Huifeng Jiang
- Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
- DOI
- https://doi.org/10.1038/s41467-019-09095-z
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 10
Abstract
The microbial synthesis of carbon-containing compounds from single carbon precursors is desirable, yet designed pathways to achieve this goal overlap with host metabolism. Here the authors design a de novo metabolic pathway to assimilate formaldehyde into acetyl-CoA that does not overlap with known metabolic networks.
