RNAs m6A modification facilitates UVB-induced photoaging

Shuping Zhang; Meng Wu; Tingting Lu; Xiaoying Tian; Lihua Gao; Siyu Yan; Dan Wang; Jinrong Zeng; Lina Tan

Heliyon (Nov 2024)

RNAs m6A modification facilitates UVB-induced photoaging

Shuping Zhang,
Meng Wu,
Tingting Lu,
Xiaoying Tian,
Lihua Gao,
Siyu Yan,
Dan Wang,
Jinrong Zeng,
Lina Tan

Affiliations

Shuping Zhang: Department of Dermatology, Postdoctoral Station of Clinical Medicine, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China
Meng Wu: Department of Dermatology, Xiangya Hospital of Central South University, Changsha, 410008, Hunan, China; Department of Dermatology, Hunan Provincial People's Hospital of Hunan Normal University, Changsha, 410005, Hunan, China
Tingting Lu: Department of Dermatology, Postdoctoral Station of Clinical Medicine, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China
Xiaoying Tian: Department of Dermatology, Postdoctoral Station of Clinical Medicine, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China
Lihua Gao: Department of Dermatology, Postdoctoral Station of Clinical Medicine, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China
Siyu Yan: Department of Dermatology, Postdoctoral Station of Clinical Medicine, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China
Dan Wang: Department of Dermatology, Postdoctoral Station of Clinical Medicine, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China
Jinrong Zeng: Department of Dermatology, Postdoctoral Station of Clinical Medicine, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China; Corresponding author.
Lina Tan: Department of Dermatology, Postdoctoral Station of Clinical Medicine, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China; Corresponding author.

Journal volume & issue: Vol. 10, no. 21
p. e39532

Abstract

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RNA N6-methylation (m6A) modification is common in eukaryotic mRNA and has been linked to various physiological disorders, including UVB-induced photoaging. To identify biological differences among photoaging. Three pairs of normal and photoaged skin tissues were collected for m6A RNA sequencing assay. Transcriptome profiles showed differential m6A methylation modifications in 1365 mRNAs in photoaging skin tissues. Pathway analysis revealed the involvement of cellular stress response and regulation of cell cycle G2/M phase transition in m6A-mRNAs. Further experiments validated the differential expression of m6A methyltransferases (METTL3 and METTL14) and hypermethylation modification in mRNAs (CENPE, PPM1B and TPM1). In vitro studies demonstrated that increased METTL3 and METTL14 levels promoted m6A methylation of CENPE, PPM1B and TPM1 in UVB-induced photoaging cells, and further experiments on mice showed that downregulation of METTL3 and METTL14 reduced m6A modifications in CENPE, PPM1B and TPM1, leading to the delayed appearance of photoaging phenotypes, suggesting that these genes could serve as potential therapeutic targets for treating photoaging. Our study characterized key transcriptome changes in photoaging and identified the role of METTL3 and METTL14 in mediating m6A modification, resulting in the upregulation of CENPE, PPM1B and TPM1 expression, which may be crucial in UVB-induced photoaging.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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