Benchmarking of SpCas9 variants enables deeper base editor screens of BRCA1 and BCL2

Annabel K. Sangree; Audrey L. Griffith; Zsofia M. Szegletes; Priyanka Roy; Peter C. DeWeirdt; Mudra Hegde; Abby V. McGee; Ruth E. Hanna; John G. Doench

doi:10.1038/s41467-022-28884-7

Nature Communications (Mar 2022)

Benchmarking of SpCas9 variants enables deeper base editor screens of BRCA1 and BCL2

Annabel K. Sangree,
Audrey L. Griffith,
Zsofia M. Szegletes,
Priyanka Roy,
Peter C. DeWeirdt,
Mudra Hegde,
Abby V. McGee,
Ruth E. Hanna,
John G. Doench

Affiliations

Annabel K. Sangree: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Audrey L. Griffith: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Zsofia M. Szegletes: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Priyanka Roy: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Peter C. DeWeirdt: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Mudra Hegde: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Abby V. McGee: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
Ruth E. Hanna: Genetic Perturbation Platform, Broad Institute of MIT and Harvard
John G. Doench: Genetic Perturbation Platform, Broad Institute of MIT and Harvard

DOI: https://doi.org/10.1038/s41467-022-28884-7
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 17

Abstract

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Numerous rationally-designed and directed-evolution variants of SpCas9 have been reported to expand the utility of CRISPR technology. Here the authors make comparisons of numerous Cas9 variants, nominate options for base editing screens with denser coverage with A>G and C>T base editing screens and identify loss-of-function mutations in BRCA1 and Venetoclax-resistant mutations in BCL2.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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