Calcium-Polyphosphate Submicroparticles (CaPP) Improvement Effect of the Experimental Bleaching Gels’ Chemical and Cellular-Viability Properties

Abstract

The aim of this research was to develop and characterize the chemical and cellular-viability properties of an experimental high-concentration bleaching gel (35 wt%-H2O2) containing calcium-polyphosphate particles (CaPP) at two concentrations (0.5 wt% and 1.5 wt%). The CaPP submicroparticles were synthesized by coprecipitation, keeping a Ca:P ratio of 2:1. The CaPP morphology, size, and chemical and crystal profiles were characterized through scanning and transmission electron microscopy, energy-dispersive X-ray analysis, and X-ray diffraction, respectively. The assessed bleaching gels were experimental (without CaPP); 0.5% CaPP; 1.5% CaPP; and commercial. The gels’ pH values and H2O2 concentrations (iodometric titration) were determined. The odontoblast-like cell viability after a gel’s exposure was assessed by the MTT assay. The pH and H2O2 concentration were compared through a repeated-measures analysis of variance (ANOVA) and a Tukey’s test and the cell viability through a one-way ANOVA and a Tukey’s test using a GraphPad Prism (α 2O2 concentration decreased in all groups after mixing (p p < 0.05). The successful incorporation of CaPP had a positive impact on the bleaching gel’s chemical and cellular-viability properties when compared to the experimental gel without these particles.

Keywords

• hydrogen peroxide
• bleaching
• cell survival
• pH
• polyphosphates
• submicroparticles