A CRISPR-based ultrasensitive assay detects attomolar concentrations of SARS-CoV-2 antibodies in clinical samples

Yanan Tang; Turun Song; Lu Gao; Saifu Yin; Ming Ma; Yun Tan; Lijuan Wu; Yang Yang; Yanqun Wang; Tao Lin; Feng Li

doi:10.1038/s41467-022-32371-4

Nature Communications (Aug 2022)

A CRISPR-based ultrasensitive assay detects attomolar concentrations of SARS-CoV-2 antibodies in clinical samples

Yanan Tang,
Turun Song,
Lu Gao,
Saifu Yin,
Ming Ma,
Yun Tan,
Lijuan Wu,
Yang Yang,
Yanqun Wang,
Tao Lin,
Feng Li

Affiliations

Yanan Tang: Key Laboratory of Green Chemistry & Technology of Ministry of Education, College of Chemistry, Analytical & Testing Center, Sichuan University
Turun Song: Urology Department, Urology Research Institute, Organ Transplantation Center, West China Hospital, Sichuan University
Lu Gao: Key Laboratory of Green Chemistry & Technology of Ministry of Education, College of Chemistry, Analytical & Testing Center, Sichuan University
Saifu Yin: Urology Department, Urology Research Institute, Organ Transplantation Center, West China Hospital, Sichuan University
Ming Ma: Urology Department, Urology Research Institute, Organ Transplantation Center, West China Hospital, Sichuan University
Yun Tan: Key Laboratory of Green Chemistry & Technology of Ministry of Education, College of Chemistry, Analytical & Testing Center, Sichuan University
Lijuan Wu: Department of Laboratory Medicine, West China Hospital, Sichuan University
Yang Yang: Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People’s Hospital, Second Hospital Affiliated to Southern University of Science and Technology
Yanqun Wang: State Key Laboratory of Respiratory Disease, National Clinical Research Centre for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University
Tao Lin: Urology Department, Urology Research Institute, Organ Transplantation Center, West China Hospital, Sichuan University
Feng Li: Key Laboratory of Green Chemistry & Technology of Ministry of Education, College of Chemistry, Analytical & Testing Center, Sichuan University

DOI: https://doi.org/10.1038/s41467-022-32371-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 10

Abstract

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CRISPR diagnostics are routinely used for the detecting nucleic acids, but rarely for clinically important proteins. Here, by translating a CRISPR-based DNA test into an ultrasensitive assay for antibodies, the authors achieve antibody detection from serum samples at attomolar concentrations.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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