Effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo (Bubalus bubalis) semen

Abstract

The present study was carried out to investigate the effect of cholesterol loaded cyclodextrin on activity of antioxidants during cryopreservation of buffalo semen. Ejaculates (48) with mass motility ≥3+ from 3 bulls (16 from each bull) were collected as per standard procedure. Each ejaculate was divided into group I (control): diluted with Tris-egg yolk-glycerol dilutor up to 60×106 sperm/ml and group II: treated with cholesterol loaded cyclodextrin @ 3 mg/120×106 sperm and incubated at 37ºC for 10 min and then finally diluted with Tris-egg yolk-glycerol dilutor up to 60×106 sperm/ml. French medium straws (0.5 ml) were filled with the extended semen samples, sealed with polyvinyl alcohol powder and kept for 3 h at 5ºC for equilibration and then kept in automatic programmable freezer till temperature of straws reached –145°C followed by plunging into liquid nitrogen (– 196°C). Ejaculates were evaluated freshly, before freezing and after thawing for seminal attributes (progressive motility, viability, acrosomal integrity and hypo-osmotic swelling response) and antioxidants (superoxide dismutase, catalase, glutathione peroxidase and total antioxidants). After thawing, activity of superoxide dismutase, catalase and total antioxidants were significantly higher in group II as compared to group I. It is concluded that cholesterol loaded cyclodextrin enhances seminal antioxidant activity.

Keywords

• Antioxidants
• Buffalo
• Cholesterol loaded cyclodextrin
• Cryopreservation
• Semen