STAR Protocols (Sep 2024)
Protocol for behavioral and neural recording in macaques during a novelty detection task
- Xinyu Du,
- Peirun Song,
- Yumei Gong,
- Yuying Zhai,
- Haoxuan Xu,
- Hangting Ye,
- Xuehui Bao,
- Qianyue Huang,
- Zhiyi Tu,
- Pei Chen,
- Xuan Zhao,
- David Pérez-González,
- Manuel S. Malmierca,
- Xiongjie Yu
Affiliations
- Xinyu Du
- Department of Anesthesia, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China; Zhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China
- Peirun Song
- Department of Anesthesia, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China; Zhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China
- Yumei Gong
- Department of Anesthesia, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China; Zhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China; Department of Anesthesiology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200000, China; Hangzhou Extremely Weak Magnetic Field Major Science and Technology, Infrastructure Research Institute, Hangzhou 310000, China; Interdisciplinary Institute of Neuroscience and Technology, College of Biomedical, Engineering and Instrument Science, Zhejiang University, Hangzhou 310000, China
- Yuying Zhai
- Department of Anesthesia, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China; Zhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China
- Haoxuan Xu
- Interdisciplinary Institute of Neuroscience and Technology, College of Biomedical, Engineering and Instrument Science, Zhejiang University, Hangzhou 310000, China
- Hangting Ye
- Department of Anesthesia, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China; Zhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China
- Xuehui Bao
- Interdisciplinary Institute of Neuroscience and Technology, College of Biomedical, Engineering and Instrument Science, Zhejiang University, Hangzhou 310000, China
- Qianyue Huang
- Interdisciplinary Institute of Neuroscience and Technology, College of Biomedical, Engineering and Instrument Science, Zhejiang University, Hangzhou 310000, China
- Zhiyi Tu
- Department of Anesthesiology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200000, China
- Pei Chen
- Department of Anesthesiology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200000, China
- Xuan Zhao
- Department of Anesthesiology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200000, China
- David Pérez-González
- Cognitive and Auditory Neuroscience Laboratory (Lab 1), Institute of Neuroscience of Castilla y León (INCYL), University of Salamanca, 37005 Salamanca, Spain; Institute for Biomedical Research of Salamanca (IBSAL), 37007 Salamanca, Spain; Department of Basic Psychology, Psychobiology and Methodology of Behavioral Sciences, Faculty of Psychology, University of Salamanca, 37007 Salamanca, Spain
- Manuel S. Malmierca
- Cognitive and Auditory Neuroscience Laboratory (Lab 1), Institute of Neuroscience of Castilla y León (INCYL), University of Salamanca, 37005 Salamanca, Spain; Institute for Biomedical Research of Salamanca (IBSAL), 37007 Salamanca, Spain; Department of Cell Biology and Pathology, Faculty of Medicine, University of Salamanca, 37007 Salamanca, Spain; Corresponding author
- Xiongjie Yu
- Department of Anesthesia, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China; Zhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310000, China; Department of Anesthesiology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200000, China; Corresponding author
- Journal volume & issue
-
Vol. 5,
no. 3
p. 103252
Abstract
Summary: The neural mechanisms of novelty detection, especially in relation to behavior, are currently poorly understood. Here, we present a protocol for recording neuronal activity in macaque auditory cortex during novelty detection tasks. We describe steps for behavioral training, surgical headpost implantation, MRI-based electrode targeting, and electrophysiological recording. These steps allow direct assessment of the correlation between novelty detection behavior and neuronal activity.For complete details on the use and execution of this protocol, please refer to Gong et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
