Сolonial–morphological characteristics of dissociatives of the Rhodococcus ruber - 8/4/1 strain

Abstract

This article provides a study of the molecular, physiological, biochemical and morphological features of R-, S- and M-dissociants of Rhodococcus ruber - 8/4/1. The sequence similarity of the genome fragments of R- and S-dissociant forms encoded by 16S rRNA was demonstrated. MALDI spectra were obtained for the R. ruber-8/4/1 strain and its dissociants. Under standard analysis conditions, the MALDI profiles of R-, S- and M- forms dissociants of the Rhodococcus ruber - 8/4/1 were similar. The determination of the resistance of the strain dissociants to external factors showed that R-forms were more stable under UV irradiation and increased temperature. The S-forms showed stability upon the conversion of acrylic acid nitrile into acrylamide (AA), whereas the M-forms were stable under a decrease in the cultivation temperature, and an increase in the concentration of NaCl in the medium. The S-forms proved to be the most active in the transformation of acrylonitrile into AA.

Keywords

• dissociation; forms; strain; Rhodococcus ruber - 8/4/1; biotransformation; nitrile hydratase.