Biomolecules (Apr 2020)

Bifunctional Non-Canonical Amino Acids: Combining Photo-Crosslinking with Click Chemistry

  • Jan-Erik Hoffmann

DOI
https://doi.org/10.3390/biom10040578
Journal volume & issue
Vol. 10, no. 4
p. 578

Abstract

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Genetic code expansion is a powerful tool for the study of protein interactions, as it allows for the site-specific incorporation of a photoreactive group via non-canonical amino acids. Recently, several groups have published bifunctional amino acids that carry a handle for click chemistry in addition to the photo-crosslinker. This allows for the specific labeling of crosslinked proteins and therefore the pulldown of peptides for further analysis. This review describes the properties and advantages of different bifunctional amino acids, and gives an overview about current and future applications.

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