In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy

Hala Zuhayri; Viktor V. Nikolaev; Anastasia I. Knyazkova; Tatiana B. Lepekhina; Natalya A. Krivova; Valery V. Tuchin; Yury V. Kistenev

doi:10.3390/pharmaceutics14020287

Pharmaceutics (Jan 2022)

In Vivo Quantification of the Effectiveness of Topical Low-Dose Photodynamic Therapy in Wound Healing Using Two-Photon Microscopy

Hala Zuhayri,
Viktor V. Nikolaev,
Anastasia I. Knyazkova,
Tatiana B. Lepekhina,
Natalya A. Krivova,
Valery V. Tuchin,
Yury V. Kistenev

Affiliations

Hala Zuhayri: Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, 36 Lenin Av., 634050 Tomsk, Russia
Viktor V. Nikolaev: Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, 36 Lenin Av., 634050 Tomsk, Russia
Anastasia I. Knyazkova: Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, 36 Lenin Av., 634050 Tomsk, Russia
Tatiana B. Lepekhina: Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, 36 Lenin Av., 634050 Tomsk, Russia
Natalya A. Krivova: Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, 36 Lenin Av., 634050 Tomsk, Russia
Valery V. Tuchin: Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, 36 Lenin Av., 634050 Tomsk, Russia
Yury V. Kistenev: Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University, 36 Lenin Av., 634050 Tomsk, Russia

DOI: https://doi.org/10.3390/pharmaceutics14020287
Journal volume & issue: Vol. 14, no. 2
p. 287

Abstract

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The effect of low-dose photodynamic therapy on in vivo wound healing with topical application of 5-aminolevulinic acid and methylene blue was investigated using an animal model for two laser radiation doses (1 and 4 J/cm2). A second-harmonic-generation-to-auto-fluorescence aging index of the dermis (SAAID) was analyzed by two-photon microscopy. SAAID measured at 60–80 μm depths was shown to be a suitable quantitative parameter to monitor wound healing. A comparison of SAAID in healthy and wound tissues during phototherapy showed that both light doses were effective for wound healing; however, healing was better at a dose of 4 J/cm2.

Published in Pharmaceutics

ISSN: 1999-4923 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Pharmacy and materia medica
Website: http://www.mdpi.com/journal/pharmaceutics/

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Abstract

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