Structure-function relationships during metaphloem sieve elements development in Triticum aestivum

Abstract

The differentiation of metaphloem sieve element (MSEs) in the developing caryopsis of wheat (Triticum aestivum L.) was a programmed cell semi-death process. We studied the changes of microtubules and polysaccharide contents during MSEs development. Some significant features are presented in MSEs, such as cell wall non-uniform thickening, chromatin condensation and so on. During the period of MSEs differentiation, numerous microtubules are distributed in the vicinity of the cell wall, but finally they vanished in mature MSEs. Large glycoconjugates in the cell wall and small glycoconjugates in the Golgi apparatus were observed in the developing MSEs. Programmed cell death (PCD) ceased in the mature MSEs after 6 d after flowering and higher aggregation of glycoconjugates appeared in the cytoplasm. All of these processes were in tight contact with the cell wall non-uniform thickening during PCD.

Keywords

• cell wall
• microtubules
• polysaccharides
• programmed cell death
• wheat