Non-synonymous single nucleotide variations of TP0265 in Guangdong clinical strain of Treponema pallidum

Abstract

Objective To compare the non-synonymous single nucleotide polymorphisms of the carrier protein of branched-chain amino acid transport system Ⅱ (TP0265) of Treponema pallidum (Tp) in Guangdong clinical strain (GD1) with the standard strain of Nichols in the United States and Treponema spp. Methods GD1 was serially subcultured in rabbit testis for obtaining Tp bacterial suspension. GD1 DNA was extracted and the concentration of GD1 genome was quantitatively detected by TP0574 qPCR. GD1 DNA was detected by TP0548 PCR and genotyping system. PCR detection by TP0265 Multiple sequence alignment analysis was performed between GD1 TP0265 and nsSNPs of American Nichols standard strain and Treponema globe. Results The sequencing results of TP0548 were confirmed to be f subtype by BioEdit multiple sequence alignment. Compared with the American Nichols strain, it was found that GD1 had a G175A mutation at the TP0265 base, resulting in the mutation of proline (P) at position 230 of the TP0265 protein to leucine (L). Compared with different Treponema species in the global Treponema genus, except for Nichols, Dal-1 and Nichols Houston in the United States, the TP0265 mutation was found. Conclusion There is a TP0265 nsSNPs mutation in GD1, which involves a wide range of Treponema species worldwide.

Keywords

• treponema pallidum
• branched-chain amino acid transport system ⅱ carrier protein
• non-synonymous single nucleotide polymorphism