Frontiers in Genetics (Sep 2022)

Integrated bioinformatics analysis for novel miRNAs markers and ceRNA network in diabetic retinopathy

  • Jingru Li,
  • Chaozhong Li,
  • Yulan Zhao,
  • Xinyu Wu,
  • Shuai Yu,
  • Guihu Sun,
  • Peng Ding,
  • Si Lu,
  • Lijiao Zhang,
  • Ping Yang,
  • Yunzhu Peng,
  • Jingyun Fu,
  • Luqiao Wang

DOI
https://doi.org/10.3389/fgene.2022.874885
Journal volume & issue
Vol. 13

Abstract

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In order to seek a more outstanding diagnosis and treatment of diabetic retinopathy (DR), we predicted the miRNA biomarkers of DR and explored the pathological mechanism of DR through bioinformatics analysis.Method: Based on public omics data and databases, we investigated ncRNA (non-coding RNA) functions based on the ceRNA hypothesis.Result: Among differentially expressed miRNAs (DE-miRNAs), hsa-miR-1179, -4797-3p and -665 may be diagnosis biomarkers of DR. Functional enrichment analysis revealed differentially expressed mRNAs (DE-mRNAs) enriched in mitochondrial transport, cellular respiration and energy derivation. 18 tissue/organ-specific expressed genes, 10 hub genes and gene cluster modules were identified. The ceRNA networks lncRNA FBXL19-AS1/miR-378f/MRPL39 and lncRNA UBL7-AS1/miR-378f/MRPL39 might be potential RNA regulatory pathways in DR.Conclusion: Differentially expressed hsa-miR-1179, -4797-3p and -665 can be used as powerful markers for DR diagnosis, and the ceRNA network: lncRNA FBXL19-AS1/UBL7-AS1-miR-378f-MRPL39 may represent an important regulatory role in DR progression.

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