No interactions among three methane inhibitors on in vitro methane production

Abstract

Two experiments (Exps.) were conducted to examine the interactions among methane inhibitors on 24 h in vitro methane production using monensin (MON), fumaric acid (FA), and bromochloromethane (BCM). Donor goats and in vitro fermentation incubations were both treated with a 50:50 concentrate:forage diet (DM basis). In Exp. 1, MON (0, 60, or 120 mg/kg DM) and FA (0, 3.44, or 6.89 mM) were used to examine their two-way interaction, and no interaction was found on methane production (P > 0.05). In Exp. 2, MON (0 or 60 mg/kg DM), FA (0 or 6.89 mM), and BCM (0 or 3.08 μM) were used to examine their three-way interaction, and no three-way interaction was observed (P > 0.05). The correlation was significant under the MON and FA treatments without BCM (r = 0.55, P < 0.05), and under the MON, FA, and BCM treatments, the correlation was also significant (r = 0.59, P < 0.05). Collectively, no two- or three-way interactions were observed among MON, FA, and BCM. Regression analyses showed that MON, FA, and BCM were independent from each other, and the inhibitory effects of the three methane inhibitors on methanogenesis to a certain range are additive.

Keywords

• Interaction
• methane inhibitors
• monensin
• fumaric acid
• bromochloromethane