Mangiferin reduces high-starch diet-induced lipid accumulation and liver damage by modulating triglyceride metabolism in largemouth bass (Micropterus salmoides)

Yutong Zheng; Qisheng Lu; Jingyue Cao; Guoli Han; Yulong Liu; Haokun Liu; Junyan Jin; Zhimin Zhang; Yunxia Yang; Xiaoming Zhu; Dong Han; Shouqi Xie

Aquaculture Reports (Aug 2024)

Mangiferin reduces high-starch diet-induced lipid accumulation and liver damage by modulating triglyceride metabolism in largemouth bass (Micropterus salmoides)

Yutong Zheng,
Qisheng Lu,
Jingyue Cao,
Guoli Han,
Yulong Liu,
Haokun Liu,
Junyan Jin,
Zhimin Zhang,
Yunxia Yang,
Xiaoming Zhu,
Dong Han,
Shouqi Xie

Affiliations

Yutong Zheng: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Qisheng Lu: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
Jingyue Cao: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
Guoli Han: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Yulong Liu: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
Haokun Liu: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Junyan Jin: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Zhimin Zhang: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Yunxia Yang: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Xiaoming Zhu: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
Dong Han: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; Hubei Hongshan Laboratory, Wuhan 430070, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding author at: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Shouqi Xie: State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; The Innovative Academy of Seed Design, Chinese Academy of Sciences, Wuhan 430072, China

Journal volume & issue: Vol. 37
p. 102266

Abstract

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High-starch diets may lead to growth retardation, liver damage and lipid accumulation in fish. Although mangiferin (MGF) can regulate triglyceride metabolism and protect liver health in mammals, studies on its role in fish are limited. The largemouth bass (Micropterus salmoides) were fed four different groups of diets, including a control diet (NCD), a high-starch diet (HSD), a high-starch diet supplemented with 100 mg/kg MGF (100 MGF) and a high-starch diet supplemented with 500 mg/kg MGF (500 MGF). After 8 weeks of feeding, weight gain and specific growth rate were significantly higher in the 500 MGF group than in the HSD group. Feeding rate was significantly higher in the 100 MGF group than in the HSD group. Feed conversion ratio was significantly higher in the 100 MGF group than in the HSD group. MGF reduced HSI, ALT, AST and liver vacuolization, compared with HSD group. In addition, MGF significantly reduced plasma GLU and TG levels and increased plasma HDL levels in largemouth bass. Compared to the HSD group, MGF significantly reduced TG levels in the liver, as well as reduced hepatic lipid droplet area. MGF significantly increased the relative expressions of glycolysis-related gene (pfk1), pentose phosphate pathway-related genes (g6pd, pgd and tktb) and mitochondrial oxidative phosphorylation-related gene (ndufa2, sdhb, sdhaf3, sdhaf4 and cox4). MGF significantly decreased the relative expressions of gluconeogenesis-related gene (pepck and g6pc). MGF significantly decreased the transcriptional levels of key relative expressions of triglyceride metabolism (srebf1), fatty acid synthesis-related genes (acc1, fasn and scd1) and triglyceride synthesis-related genes (gpat2, agpat2, agpat3, agpat5, dgat1a and dgat2). MGF significantly increased the relative expressions of triglyceride catabolism-related genes (pnpla2 and lipeb). The results indicated that MGF reduced lipid accumulation and alleviated high starch diet-induced liver damage by regulating triglyceride metabolism in largemouth bass.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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