NOTCH signaling specifies arterial-type definitive hemogenic endothelium from human pluripotent stem cells

Gene I. Uenishi; Ho Sun Jung; Akhilesh Kumar; Mi Ae Park; Brandon K. Hadland; Ethan McLeod; Matthew Raymond; Oleg Moskvin; Catherine E. Zimmerman; Derek J. Theisen; Scott Swanson; Owen J. Tamplin; Leonard I. Zon; James A. Thomson; Irwin D. Bernstein; Igor I. Slukvin

doi:10.1038/s41467-018-04134-7

Nature Communications (May 2018)

NOTCH signaling specifies arterial-type definitive hemogenic endothelium from human pluripotent stem cells

Gene I. Uenishi,
Ho Sun Jung,
Akhilesh Kumar,
Mi Ae Park,
Brandon K. Hadland,
Ethan McLeod,
Matthew Raymond,
Oleg Moskvin,
Catherine E. Zimmerman,
Derek J. Theisen,
Scott Swanson,
Owen J. Tamplin,
Leonard I. Zon,
James A. Thomson,
Irwin D. Bernstein,
Igor I. Slukvin

Affiliations

Gene I. Uenishi: Wisconsin National Primate Research Center, University of Wisconsin
Ho Sun Jung: Wisconsin National Primate Research Center, University of Wisconsin
Akhilesh Kumar: Wisconsin National Primate Research Center, University of Wisconsin
Mi Ae Park: Wisconsin National Primate Research Center, University of Wisconsin
Brandon K. Hadland: Department of Pediatrics, University of Washington School of Medicine
Ethan McLeod: Wisconsin National Primate Research Center, University of Wisconsin
Matthew Raymond: Wisconsin National Primate Research Center, University of Wisconsin
Oleg Moskvin: Wisconsin National Primate Research Center, University of Wisconsin
Catherine E. Zimmerman: Wisconsin National Primate Research Center, University of Wisconsin
Derek J. Theisen: Wisconsin National Primate Research Center, University of Wisconsin
Scott Swanson: Morgridge Institute for Research
Owen J. Tamplin: Department of Pharmacology, University of Illinois
Leonard I. Zon: Stem Cell Program and Division of Hematology/Oncology, Children’s Hospital Boston, Harvard Medical School and Howard Hughes Medical Institute
James A. Thomson: Morgridge Institute for Research
Irwin D. Bernstein: Department of Pediatrics, University of Washington School of Medicine
Igor I. Slukvin: Wisconsin National Primate Research Center, University of Wisconsin

DOI: https://doi.org/10.1038/s41467-018-04134-7
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 14

Abstract

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It is unclear whether arterial specification is required for hematopoietic stem cell formation. Here, the authors use a chemically defined human pluripotent stem cell (hPSC) differentiation system to show the role of NOTCH signaling in forming arterial-type hemogenic endothelial cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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