Journal of Extracellular Vesicles (Aug 2015)
Meeting report: discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium
- Louise C. Laurent,
- Asim B. Abdel-Mageed,
- P. David Adelson,
- Jorge Arango,
- Leonora Balaj,
- Xandra Breakefield,
- Elizabeth Carlson,
- Bob S. Carter,
- Blanca Majem Cavaller,
- Clark C. Chen,
- Emanuele Cocucci,
- Kirsty Danielson,
- Amanda Courtright,
- Saumya Das,
- Zakaria Y. Abd Elmageed,
- Daniel Enderle,
- Alan Ezrin,
- Marc Ferrer,
- Jane Freedman,
- David Galas,
- Roopali Gandhi,
- Matthew J. Huentelman,
- Kendall Van Keuren-Jensen,
- Yashar Kalani,
- Yong Kim,
- Anna M. Krichevsky,
- Charles Lai,
- Madhu Lal-Nag,
- Clara D. Laurent,
- Trevor Leonardo,
- Feng Li,
- Ivana Malenica,
- Debasis Mondal,
- Parham Nejad,
- Tushar Patel,
- Robert L. Raffai,
- Renee Rubio,
- Johan Skog,
- Robert Spetzler,
- Jie Sun,
- Kahraman Tanriverdi,
- Kasey Vickers,
- Liang Wang,
- Yaoyu Wang,
- Zhiyun Wei,
- Howard L. Weiner,
- David Wong,
- Irene K. Yan,
- Ashish Yeri,
- Stephen Gould
Affiliations
- Louise C. Laurent
- Division of Maternal Fetal Medicine, Department of Reproductive Medicine, University of California San Diego, San Diego, CA, USA
- Asim B. Abdel-Mageed
- Department of Urology, Tulane University, School of Medicine, New Orleans, LA, USA
- P. David Adelson
- Phoenix Children's Hospital, Phoenix, AZ, USA
- Jorge Arango
- Phoenix Children's Hospital, Phoenix, AZ, USA
- Leonora Balaj
- Department of Neurology, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA
- Xandra Breakefield
- Department of Neurology, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA
- Elizabeth Carlson
- Neurogenomics Division, Translational Genomics Research Institute, Phoenix, AZ, USA
- Bob S. Carter
- Center for Theoretical and Applied Neuro-Oncology, Division of Neurosurgery, University of California San Diego, San Diego, CA, USA
- Blanca Majem Cavaller
- School of Dentistry, University of California, Los Angeles, CA, USA
- Clark C. Chen
- Center for Theoretical and Applied Neuro-Oncology, Division of Neurosurgery, University of California San Diego, San Diego, CA, USA
- Emanuele Cocucci
- Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA
- Kirsty Danielson
- Cardiovascular Institute, Beth Israel Deaconess Medical Center, Boston, MA, USA
- Amanda Courtright
- Neurogenomics Division, Translational Genomics Research Institute, Phoenix, AZ, USA
- Saumya Das
- Cardiovascular Institute, Beth Israel Deaconess Medical Center, Boston, MA, USA
- Zakaria Y. Abd Elmageed
- Department of Urology, Tulane University, School of Medicine, New Orleans, LA, USA
- Daniel Enderle
- Exosome Diagnostics GmbH, Martinsried, Germany
- Alan Ezrin
- NXPharmaGen, Miami, FL, USA
- Marc Ferrer
- Division of Preclinical Innovation, National Center for Advancing Translational Sciences (NCATS), National Institutes of Health, Bethesda, MD, USA
- Jane Freedman
- Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA
- David Galas
- Pacific Northwest Diabetes Research Institute, Seattle, WA, USA
- Roopali Gandhi
- Department of Neurology, Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
- Matthew J. Huentelman
- TGen, Phoenix, AZ, USA
- Kendall Van Keuren-Jensen
- Neurogenomics Division, Translational Genomics Research Institute, Phoenix, AZ, USA
- Yashar Kalani
- Barrow Neurological Institute, Phoenix, AZ, USA
- Yong Kim
- School of Dentistry, University of California, Los Angeles, CA, USA
- Anna M. Krichevsky
- Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
- Charles Lai
- Department of Neurology, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA
- Madhu Lal-Nag
- Division of Preclinical Innovation, National Center for Advancing Translational Sciences (NCATS), National Institutes of Health, Bethesda, MD, USA
- Clara D. Laurent
- Cardiovascular Institute, Beth Israel Deaconess Medical Center, Boston, MA, USA
- Trevor Leonardo
- Cardiovascular Institute, Beth Israel Deaconess Medical Center, Boston, MA, USA
- Feng Li
- School of Dentistry, University of California, Los Angeles, CA, USA
- Ivana Malenica
- Neurogenomics Division, Translational Genomics Research Institute, Phoenix, AZ, USA
- Debasis Mondal
- Department of Pharmacology, Tulane University School of Medicine, New Orleans, LA, USA
- Parham Nejad
- Department of Neurology, Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
- Tushar Patel
- Department of Transplantation, Mayo Clinic Florida, Jacksonville, FL, USA
- Robert L. Raffai
- Department of Surgery, University of California San Francisco, San Francisco, CA, USA
- Renee Rubio
- Center for Cancer Computational Biology, Dana Farber Cancer Institute, Boston, MA, USA
- Johan Skog
- Exosome Diagnostics, Cambridge, MA, USA
- Robert Spetzler
- Barrow Neurological Institute, Phoenix, AZ, USA
- Jie Sun
- School of Dentistry, University of California, Los Angeles, CA, USA
- Kahraman Tanriverdi
- Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA
- Kasey Vickers
- Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN, USA
- Liang Wang
- Department of Pathology, Medical College of Wisconsin, Milwaukee, WI, USA
- Yaoyu Wang
- Center for Cancer Computational Biology, Dana Farber Cancer Institute, Boston, MA, USA
- Zhiyun Wei
- Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
- Howard L. Weiner
- Department of Neurology, Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
- David Wong
- School of Dentistry, University of California, Los Angeles, CA, USA
- Irene K. Yan
- Department of Cancer Biology, Mayo Clinic Florida, Jacksonville, FL, USA
- Ashish Yeri
- Neurogenomics Division, Translational Genomics Research Institute, Phoenix, AZ, USA
- Stephen Gould
- Department of Biological Chemistry, Johns Hopkins University, Baltimore, MD, USA
- DOI
- https://doi.org/10.3402/jev.v4.26533
- Journal volume & issue
-
Vol. 4,
no. 0
pp. 1 – 18
Abstract
Extracellular RNAs (exRNAs) have been identified in all tested biofluids and have been associated with a variety of extracellular vesicles, ribonucleoprotein complexes and lipoprotein complexes. Much of the interest in exRNAs lies in the fact that they may serve as signalling molecules between cells, their potential to serve as biomarkers for prediction and diagnosis of disease and the possibility that exRNAs or the extracellular particles that carry them might be used for therapeutic purposes. Among the most significant bottlenecks to progress in this field is the lack of robust and standardized methods for collection and processing of biofluids, separation of different types of exRNA-containing particles and isolation and analysis of exRNAs. The Sample and Assay Standards Working Group of the Extracellular RNA Communication Consortium is a group of laboratories funded by the U.S. National Institutes of Health to develop such methods. In our first joint endeavour, we held a series of conference calls and in-person meetings to survey the methods used among our members, placed them in the context of the current literature and used our findings to identify areas in which the identification of robust methodologies would promote rapid advancements in the exRNA field.
Keywords
