Communications Biology (Nov 2021)

Cycloalkane-modified amphiphilic polymers provide direct extraction of membrane proteins for CryoEM analysis

  • Anna J. Higgins,
  • Alex J. Flynn,
  • Anaïs Marconnet,
  • Laura J. Musgrove,
  • Vincent L. G. Postis,
  • Jonathan D. Lippiat,
  • Chun-wa Chung,
  • Tom Ceska,
  • Manuela Zoonens,
  • Frank Sobott,
  • Stephen P. Muench

DOI
https://doi.org/10.1038/s42003-021-02834-3
Journal volume & issue
Vol. 4, no. 1
pp. 1 – 9

Abstract

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Higgins et al. present a cycloalkane-modified amphiphilic polymer that can provide direct extraction of membrane proteins for Cryo-EM analysis. They show its utility by extracting and solving the structure of AcrB to a high resolution of 3.2 Å by single particle cryo-EM.