The effect of Alloxan induced diabetes on baseline comet test in male laboratory mice fed on different feeding systems

Abstract

Background: The comet analysis (electromagnetic relay analysis of a single cell) is a highly sensitive method for determining DNA damage because to exposure to carcinogens and other substances that consequently affect fertility. Objective: the aim of this study is to Evaluation the broken DNA in male diabetic mice. Created by alloxan and knowledge of the effect of some nutritional systems on the treatment and repair of genetic material due to diabetes. Materials and methods: In this study were used, 42 male Albino mice labs at the age of 2-3 months and weight (25-30) g then 6 animals were isolated to represent the control group, The remaining animals were injected between the thighs by alloxan 150 mg / kg and left the next day to make sure hit by diabetes and randomly distributed to six groups in addition to a set of Not affected control transactions. Results and conclusion: It was found from the results of the statistical analysis shown in figure(1) showed the presence of the highest percentage of injuries in the genetic material that was in the group of animals with diabetes and untreated for the duration of the experiment, as it was clear from the photos of this group that the tail length resulting from the migration of the genetic material DNA to outside the nucleus because to its damage, as well as the results showed that the group of animals affected by diabetes and the treatment by adding the Salvia officinalis plant to both plant and animal nutrition had a significant decrease (<0.05> P) in the proportion of genetic material injuries where the proportion of high (long guilt) infections was low in these two The two groups compared to the group of animals with diabetes and untreated for the duration of the experiment.

Keywords

• Diabetes,
• the basal comet test
• Salvia officinalis
• nutritional systems