Journal of Integrative Bioinformatics (Mar 2015)
Online High-throughput Mutagenesis Designer Using Scoring Matrix of Sequence-specific Endonucleases
Abstract
CRISPR Cas9 and other sequence-specific endonucleases are fundamental genome editors supporting gene knockout and gene therapy. A speedy and accurate computational allele designer is required for a high through-put gene mutagenesis pipeline using these new techniques.