Different Phenolic Extracts of Oil Palm Fruits and Caffeic Acid Prevent Palmitic Acid-Induced Lipotoxicity in HepG2 Cells via Improving Mitochondrial Function

Abstract

Oil palm (Elaeis guineensis Jacq.) fruits are rich in antioxidative phenolic compounds, which exert various health improving effects. This study aimed to comparatively study the protective effects of different phenolic fractions from the fruits treated with or without ultrahigh pressure (UHP) and their most abundant phenolic substance, caffeic acid, against the palmitic acid-induced lipotoxicity in HepG2 cells and to clarify the potential mechanisms. Results showed that the TG accumulation, intracellular reactive oxygen species content, cell apoptosis, and mitochondrial membrane potential depolarization were relieved by the administration of those phenolic fractions and caffeic acid. The protection of insoluble-bound (IB) phenolic fraction was the best, and UHP treatment significantly enhanced its protective effect on lipotoxicity. The lipotoxicity preventive effect may be achieved by alleviating intracellular oxidative stress, downregulating the expression of P-P38, COX-2, and iNOS to relieve inflammation, and downregulating Bax and cytochrome C and upregulating Bcl-2 to suppress the mitochondrial-mediated apoptosis. Therefore, the oil palm fruits phenolic fractions, especially the IB phenolic fraction, might be utilized as dietary antioxidants to relieve the adverse effects of a high-fat diet on the body, and UHP treatment is a potential method to increase this bioactivity.